SliceandExplantCultureProtocols–Hevnerlab2002
for axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr before dissections:1. Prepare 1X Krebs (1000 ml) and cool on ice. 2. Prepare 100 ml of sterile filtered Krebs buffer and cool on ice. 3. Prepare 100 ml serum–supplemented medium, and place in incubator set at 37°C and 5% CO2. 4. Prepare 100 ml serum–fre......閱讀全文
Slice－and－Explant－Culture－Protocols－–－Hevner－lab－2002
for axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr be
Human－Embryonic－Stem－(ES)－Cell－Protocols——General－notes－on－ES－cell－culture
hES media has a two week shelf life.hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended because i
顯微鏡技術——電子顯微技術
The Transmission Electron Microscope (TEM)?(HEI)An explanation of how the TEM works.??TEM Specimen Preparation?(HEI)??Serial Sectioning?(Walter Steffe
CDNA文庫
?CDNA文庫（主要內容如下）·?????????Construction of cDNA Library·?????????Construction of Genome DNA Library·?????????Library Screening??OthersConstruction of cD
流式細胞儀技術專輯
Flow Cytometry Analysis?(Springer Lab, Harvard University)?Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
流式細胞儀技術專輯
?最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯：?嗚咽分享到：??????Flow Cytometry Analysis?(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures?(Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures?(Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
LCM－PROTOCOLS
Slide SectioningParaffin blocks-?For?DNA?analysis:Special LCM processing schedule is followed.The water bath is cleaned using RNAse Zap?, rinsed thoro
M13噬菌體
·?????????M13 Phage?(Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
ORGANOTYPIC－LIMB－CULTURES
-embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c.-limb buds are microdissected and placed in holding medium (L15 medium suppleme
ORGANOTYPIC－KIDNEY－CULTURES
-embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c.-metanephroi and associated ureteric buds are microdissected and placed
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous?flagella - the bacterial cell has a single flagellaperitrichous?flagella - the bacterial cell has several flagella w
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes?and?Juan Carlos Zú?iga-Pflücker1Sunn
CGH－Protocols－（三）
Hybridizationreagents:?labeled tumor and normal-DNA (see protocol Nick translation)?salmon sperm DNA, 10 mg/ml (e.g. Promega)?human Cot1 DNA, 1 mg/ml
Smolke:Protocols/Western
OverviewBlotting for large V5-tagged proteins in?S. cerevisiaeMaterialsY-PER (Pierce)Halt EDTA-free Protease Inhibitor (Pierce)NuPAGE Novex Bis-Tris 4
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Streptomyces:Protocols/Conjugation
Intergeneric Conjugation and OverlayDescription?Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipient str
General－Cloning－Protocols
Large Scale Preps:?(See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 μg/mL) culture in early a.m. w
CGH－Protocols－（四）
CGH Image acquisitionImages were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss,
Western－Blotting－Protocols
back to topProtocolStandard vs. Rapid Immunodetection ProceduresThere are two types of protocols for immunodetection: Standard and rapid.Standard vs.
Streptomyces:Protocols/PCR
Description?Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template doubl
DAPI－Counterstaining－Protocols
實驗概要The ?blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; ?it appears to associate with AT clusters in the minor groove. Binding
CGH－Protocols－（二）
DNA preparation by cryotom tissue dissectionPreparations/Materials:?Cool cryostat down to -20 to -30°C about 3 hours prior to dissection?Label eppendo
CGH－Protocols－（一）
Metaphase chromosome preparationMaterials:?RPMI 1640 medium?fetal calf serum (FCS), 20%?Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best
Sectioning－stained－embryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better proto
Lactobacillus－culture
OverviewGeneral overview and guidelines on how to grow up a culture of LactobacillusMaterialsMRS broth (difco)MRS agar (difco)anaerobic conditionsProc
Streptomyces:Protocols/Transformation－by－Electroporation
Description?Transform?E.coli?cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into?E.coli).Approx. Duration:Prep
Rat－Blood－Collection－Protocols
實驗概要The procedure presented below describes a method for collecting rat blood.實驗步驟Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis?I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA.?EmbeddingB.?CuttingC.?StainingII. Pr