AntigenRetrieval
實驗概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunately, this process can result in the cross-linking of proteins which can mask binding sites on the antigen of interest. In order to unmask, or retrieve, these cross-linked sites, scientists can utilize a variety of procedures known collectively as antigen retri......閱讀全文
Antigen－Retrieval
實驗概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortun
Antigen－Retrieval
實驗概要Formalin ?fixed paraffin embedded tissue is a very common preparation for a wide ?variety of tissue types used in immunohistochemistry. Unfortunat
Antigen－Processing－and－Presentation
Invariant chain (Ii) associates with newly synthesized class II molecules in the endoplasmic reticulum (ER), an interaction that has been shown to int
什么是抗原(Antigen)－？
凡能刺激機體產生抗體，并能與抗體發生特異性結合的物質稱為抗原。物質所具有的這種特性稱為抗原性。抗原的物質可以是機體自身的成分如蛋白、酶、多肽或者多糖。也有外來的抗原（如病毒、污染物）。?抗原的特異性：各種抗原物質的化學組成雖然很復雜，但能刺激機體產生抗體并與抗體反應相結合的化學組成，僅僅是抗原物質表
Antigen－Affinity－Purification－of－Antibodies
實驗概要To acquire purified antibodies (This method typically yields >95% pure specific antibodies ).實驗原理?Cytokines ?are signaling proteins necessary for
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
癌胚抗原(Carcinombryonic－antigen－,CEA)
自1965年Gotel等從結腸癌分離出一種腫瘤的相關抗原后，經過進一步的研究后發現，它存在于胎兒的腸里，由于人們將其稱為癌胚抗原。在實際應用 中，CEA是一種廣譜的標記物。經實驗證明，它對胃癌，腸癌，肺癌，乳腺癌，胰腺癌，子宮頸癌，子宮體腺癌，卵巢癌，皮膚鱗狀細胞癌，膀胱移行上皮癌，食 道癌
Antigen－Dependent－B－Cell－Activation
A key part of the immune system is the production of immunoglobulins (antibodies) by B cells to bind and inactivate specific foreign antigens. The bod
Immunohistochemistry－on－paraffin－embedded－sections
主要試劑1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
ATATES－Genomic－Antigen－Immunotherapy－for－Potential－Cure－of－Cancer
分析測試百科網訊 2015年10月20 日，第十三屆國際新藥發明科技年會與第五屆藥物遞放系統研討會（SDDS-2015）于北京希爾頓逸林酒店同期舉辦并開幕。 BioMedicure公司總裁 錢勇 BioMedicure公司總裁兼首席執行官錢勇帶來題目為《ATATES Genomic
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Immunohistochemistry
實驗概要The following ?procedure describes the application of peroxidase or alkaline ?phosphatase conjugates in the immunohistochemical labeling of ?forma
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.實驗步驟The following protocol used B6 mice
Microwave－citrate－Pretreatment－of－Paraffin－Sections
1.Deparaffinize slides a (after drying thoroughly overnight at RT) in 2 changes of xylene (or xylene substitute) for 10 mins each.2.Transfer slides to
組織學——微波技術
Microwave Processing Techniques for Microscopy?(Energy Beam Sciences, Inc.)Introduction to microwave technique and detailed protocol for its applicati
immunofluorescence－of－rabbit－antimurine－VEGF－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.實驗步驟The following protocol used normal muri
可溶性抗原(soluble－antigen)的制備及鑒定
蛋白質、糖蛋白、脂蛋白、酶類、補體、脂多糖、細菌外毒素和核酸等均為可溶性抗原，它們有相當部分來源于組織和細胞，成分復雜。制備這類免疫原時，首先須將組織和細胞破碎，然后再從組織和細胞勻漿中提取目的蛋白或其他抗原，提純的抗原需鑒定后才能用做免疫原。?一、組織勻漿的制備用于制備免疫原的材料必須是新鮮或低溫
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech.實驗步驟The following protocol used Trichur
Immunohistochemistr...
實驗概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human RANTES.實驗步驟The following protocol used formalin-fixed paraffin-embedded hu
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.實驗步驟The following protocol used B6 mice
immunohistochemistry－of－mouse－antihuman－Eotaxin2－by－Peprotech
實驗概要The following protocol provides a method of immunohistochemistry of mouse anti-human Eotaxin-2 by Peprotech.實驗步驟The following protocol used formal
Immunohistochemistr...
實驗概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human MCP-1*.實驗步驟The following protocol used formalin-fixed paraffin-embeddedhum
immunofluorescence－of－mouse－antihuman－IL6－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of mouse anti-human IL-6 by Peprotech.實驗步驟The following protocol used human endomet
免疫組織化學
· ????????Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens?(KPL)·?????????Immunohistochemistry?(Tyner lab)This is a
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用說明
Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理小鼠乙型肝炎e抗原(HBeAg)ELISA試劑盒貨號:FK-QZ2967規格：96T/48TMouse hepatitis B virus e antigen,HBeAg ELISA Kit
TUNEL－assay
PROTOCOL:?Deparaffinize and rehydrate slides:3 x 3′ Xylene3 x 2′ 100% ethanol1 x 2′ 95%, 80%, 70% ethanol (each)1 x 5′ 1x PBS?Microwave antigen retrie
Preparing－cells－and...
實驗概要The method provides a protocol and tips for BrdU staining in tissue sections.Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用說明書
供應商： 上海樊克生物科技有限公司 數量： 100 規格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA試劑盒 貨號:FK-QZ2967
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用說明書
供應商： 上海樊克生物科技有限公司 數量： 100 規格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA試劑盒 貨號:FK-QZ2967
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用說明書
供應商： 上海樊克生物科技有限公司 數量： 100 規格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA試劑盒 貨號:FK-QZ2967