Vybrant?DyeCycle?GreenandOrangeStains
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired c......閱讀全文
DNA－Cell－Cycle
Solutions70% ethanolribonuclease (100 μg/ml DNase free, Sigma)propidium iodide ( 50 μg/ml in PBS)ProcedureHarvest cells. Spin at 1200 rpm for 5 minute
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Cyclins－and－Cell－Cycle－Regulation
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted f
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
The－SARScoronavirus－Life－Cycle
Severe Acute Respiratory Syndrome (SARS) has affected thousands of individuals, causing fever, and pneumonia with a mortality rate estimated at 10-18%
Cell－Cycle－Staining－ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
光合碳循環－（photosynthetic－carbon－cycle）
光合作用中碳同化（二氧化碳轉化為糖或其磷酸酯）的基本途徑。又稱卡爾文循環、還原戊糖磷酸循環、還原戊糖磷酸途徑。在綠色植物、藍藻和多種光合細菌中普遍存在。其他碳同化途徑如 C4 途徑和 CAM途徑（見景天科酸代謝）所固定的 CO2 ，最終仍須通過光合碳循環才能被還原成糖。因此它是地球上絕大部分有機物
BTG－family－proteins－and－cell－cycle－regulation
BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
Cell－cycle－analysis－of－Escherichia－coli－cells
Cell cycle analysis of?Escherichia coli?cellsC period = the time for a round of chromosome replicationD period = the time between the end of a round o
The－reactions－that－feed－amino－groups－into－the－urea－cycle
Excess amino acids in the body can be used as a source of energy, with their carbon skeleton converted to metabolic intermediates such as acetyl-CoA o
PTEN－dependent－cell－cycle－arrest－and－apoptosis
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
Regulation－of－cell－cycle－progression－by－Plk3
The focus of this pathway is to illustrate the role of Polo-like Kinase 3 (Plk3 also known as Prk and Fnk) as part of the regulatory cascade leading t
Cycle－Sequence－Reactions－For－Large－Insert－Plasmid－Templates
The following dye-labeled terminator reaction chemistries have been designed to balance conservation of reagents with the resulting sequence product s
Cell－Cycle:－G2/M－Checkpoint
The G2/M DNA damage checkpoint prevents the cell from entering mitosis (M phase) if the genome is damaged. The Cdc2-cyclin B kinase is pivotal in regu
Gammaaminobutyric－Acid－Receptor－Life－Cycle
Gamma-amino butyric acid (GABA) is an inhibitory neurotransmitter that acts at synapses through binding to ligand-gated ion channels, the GABA recepto
Cell－Cycle：細胞永生化研究進展
每一天，你身體內的一些細胞會停止分裂，這是一件好事。而有些細胞會無限增殖，這是大多數惡性腫瘤發展的一個重要早期步驟。 盡管細胞無限增殖在癌癥中有著非常的重要性，但研究人員一直對于細胞永生化背后的分子機制知之甚少。這是因為科學家缺乏好方法研究永生化人類細胞。在發表在Cell Cycle 雜志上的
Cell－Cycle:－G1/S－Check－Point
The G1/S cell cycle checkpoint controls the passage of eukaryotic cells from the first 'gap' phase (G1) into the DNA synthesis phase (S). Two
Cell－Cycle－－J－Neurosci：脊髓損傷引發大腦損害
很多脊髓損傷的研究都集中于揭示脊髓損傷所引發的效應，然而往往在研究中科學家們很容易忽視脊髓損傷對大腦所引發的效應。近日，來自馬里蘭大學的研究人員通過研究首次揭示，脊髓損傷（SCI）可以促進廣泛和持續的大腦炎癥，進而引發神經細胞的逐漸缺失以及認知問題和抑郁癥等。 相關研究發表于國際雜志Journ
Regulation－of－p27－Phosphorylation－during－Cell－Cycle－Progression
p27/Kip1 regulates the cell cycle by inhibiting the checkpoint kinase cdk2/cyclin E and blocking cell cycle progression through the G1-S transition. C
Sonic－Hedgehog－(SHH)－Receptor－Ptc1－Regulates－cell－cycle
Sonic Hedgehog (Shh) is a secreted protein identified genetically as an important developmental factor. Shh provides a morphogenic signal in the devel
Estrogenresponsive－protein－Efp－controls－cell－cycle－andbreast－tumors－growth
The estrogen-inducible RING finger protein Efp stimulates proliferation of breast cancer cells. 14-3-3 is p53-inducible protein that sequesters mitoti
細胞周期試劑盒Cell－cycle－staining－Kit中文操作步驟
試劑盒特點：1、獨創的活細胞一步法檢測，收集——洗滌——染色，簡單快速 ? 2、兼容固定細胞檢測，改進的固定方法，更少細胞粘連，更小CV值 ? 3、兼容流式和熒光顯微鏡分析，提供詳細protocol固定細胞處理：1、收集細胞懸液，細胞數為2 × 10^5 ~ 1 × 10^6。離心沉淀細胞，去上清，
Cell－Cycle：乳腺癌細胞耐藥性的分子機理
近日，來自Norris Cotton綜合癌癥研究中心的研究人員通過研究開發了一種治療ERBB2（人類表皮生長因子受體II）陽性乳腺癌的新型療法，該類乳腺癌通常會對療法產生強烈的耐藥性，相關研究刊登于國際雜志Cell Cycle上，該研究為揭示新型的癌癥耐藥機制提供了一定的線索。 研究者Kuro
讓摩爾定律一再放緩－晶圓廠的cycle－time是什么？（二）
Dai Nippon Printing（DNP）的研究員Naoya Hayashi說：“TAT更長的原因是寫入時間、檢查時間和驗證時間。”寫入時間是罪魁禍首。如上所述，IC設計要轉換為文件格式。該格式被轉換成電子束掩膜寫入器的一組指令。這個過程稱為掩膜數據準備（MDP）。然后，電子束掩膜寫入
讓摩爾定律一再放緩－晶圓廠的cycle－time是什么？（二）
對于切割，芯片制造商使用SADP/SAQP，或雙重曝光工藝。雙重曝光有時被稱為曝光-刻蝕-曝光-刻蝕（LELE）。三重曝光包括LELELE。對于多重曝光中，7nm工藝所進行沉積、蝕刻和清潔步驟是16nm/14nm的兩倍。 Coventor首席技術官David Fried表示：“隨著我
讓摩爾定律一再放緩－晶圓廠的cycle－time是什么？（一）
?從平面器件到finFET的轉變使得芯片制造商能夠將工藝和器件從16nm/14nm向更密集的方向發展，但是行業在每個節點處都面臨諸多挑戰。成本問題和技術問題都是明顯的挑戰。此外，cycle time也在逐漸增加，這是芯片尺寸縮小公式中的一個關鍵但很少宣傳的因素，這為芯片制造商和客戶帶來了更多
生物鐘基因Cycle調控家蠶滯育與生活史多樣性機制
2025年5月30日，中國科學院分子植物科學卓越創新中心詹帥研究團隊在國際學術期刊Science發表了題為“Functional polymorphism of CYCLE underlies diapause variation in moths”的研究論文。研究發現核心生物鐘基因Cycle是調控