TheEffectsofCalciumFreeSeawaterontheDevelopmentofSeaUrchinEmbryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and at the initiation of gastrulationIntroductionCalcium is necessary in two processes shortly following fertilization. One process, the acrosomal reaction, is the fusion of the acrosomal vesicle and the sperm plasma membrane......閱讀全文
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
The－effect－of－lithium－chloride－on－Lytechinus－variegates－embryos
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Solutions－for－Sea－Urchin－Development
Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literS
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish,?Danio rerio?development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Immunofluorescent－staining－of－Sea－Urchin－embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 1
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Separation－of－sea－urchin－blastomeres
Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos.Procedure:?We extractrf the g
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective:?This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos.INTRODUCTION: When th
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of?AP substrate buffer?and tu
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish,?Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process?called epiboly. Epiboly, as defined i
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
多功能胚胎激光破膜儀（二）
A new approach to cyropreservation of large equine embryos by vitrification after blastocoel micromanipulation.J. Scherzer, R. A. Fayrer-Hosken, L. Ra
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Inhibition－of－the－fast－and－slow－blocks－to－polyspermy
Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release compon
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Sea－Urchin－Fertilization
Gametes?are collected as?described. To remove some of their jelly coat,wash eggs several times in ASW by allowing them to settle and gently pouring of
Sea－Urchin－staging－series
Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their